Codon Optimization Increases Steady-State mRNA Levels in Aspergillus oryzae Heterologous Gene Expression
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چکیده
منابع مشابه
Codon Preference Optimization Increases Heterologous PEDF Expression
Pigment epithelium-derived factor (PEDF) is widely known for its neurotrophic and antiangiogenic functions. Efficacy studies of PEDF in animal models are limited because of poor heterologous protein yields. Here, we redesigned the human PEDF gene to preferentially match codon frequencies of E coli without altering the amino acid sequence. Following de novo synthesis, codon optimized PEDF (coPED...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2008
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.01354-08